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1 Mixtures and Solutions Candy Chromotography telescope Information and Research Biochemists often give this method of separation when studying amino group acids or other chemicals from living things. in any(prenominal) case they design it to separate a termination with multiple components with distinct polarites. People use chromoography to find the things in milk,find out what chemicals ar in black ink, also liberalisationrict whether something is hazardous or not. Purpose The intention of this experiment is to see what kindhearted of sullys and colourize be in the edulcorates cloaked coating. Also to see what kind of things are in it.Materials Candy with a colourationed coating, like Skittles or M&038Ms (4 different colors) corrasion alcohol or isopropyl alcohol c moroseee bean filters (2) Tall starteres or fictile cups (2) Pencil Ruler magnetic tape indulge or penning plate put over saltiness Water Toothpicks or cotton wool mop ups (4) Measur ing cups/spoons Clean pitcher or 2-liter bottle Procedure 1. Coffee filters usually are round, but its easier to compare your results if the paper is self-coloured. So your first task is to compact the coffee filter into a square measuring three cm by nine cm square from a coffee filter.You bequeath need dickens of these disinvests to complete the testing ground. 2. Space quatern drops of peeing system (or however many colors you are tryouting) equally distant on a piece of foil or plate. positioning one candy of all(prenominal) color on the drops. Give the color astir(predicate) a minute to count off into the urine. Dispose of the candy. 3. Using a pencil (do not use a pen), recede a line one cm (1/2 inch) from the edge of one last of one strip of paper. 4. Make four pencil deportations (one for to distributively one color of candy you pass on be examining) along this line, approximately 0. cm (1/4 inch) apart. Underneath all(prenominal) pane of water ice , science label the color of the candy you bequeath test on that spot. You wont tolerate set to write the whole color name, so try abbreviations like B for blue, G for green, etc. 5. Before you complete the suspire of the lab, make a prediction of the components you give birth to see from apiece candy color. 6. dowse a toothpick or cotton swab into one sample of faded water and dab the color onto the pencil dot for that color. Use a clean toothpick for each color. Try to keep each dot as small as possible. dispense with the filter paper to dry, and then go back and add more color to each dot, repeating this step a total of three condemnations for each color. 7. Prepare the salt dissolvent by mixing 1/8 teaspoon of salt and three cups of water in a clean pitcher or 2-liter bottle. Stir or shake the dissolvent until it is dissolved. This will produce a 1% salt upshot. 8. Pour the salt result into a clean tall glass so that the naiant level is 1/4 inch (0. 5 cm). 9. Tape the strip to a pencil and rest the pencil on top of the shiver so that the strip hangs into the jar.The goal is to have the end of the chromatography strip just touch sensation the surface of the solvent solution, with the colored dots preceding(prenominal) the surface of the liquid. Make sure that the colored spots do not come in direct contact with the liquid in the bottom of the glass. 10. Capillary proceeding will draw the salt solution up the paper. As it passes through the dots, it will begin to separate the dyes. When the salt water is 1/4 inch (0. 5 cm) from the top edge of the paper, remove it from the glass and place it on a clean, flatcar surface to dry. 11.Repeat steps two through nine ( utilise the second strip of paper and a clean glass) to test the same four colors using the alcohol instead of the saltwater solution at the bottom of the glass. Data and Observations airplane pilot Predictions The red M&038M will travel the farthest and be the strongest. The T he dye in the yellow M&038M will travel less and be the lightest color. pic Other observations I noticed was the unmoving phase matters. Both the red and yellow travveld to the front but had little separation. rosy-cheeked showed the lowest travelling distance.Discussion and destruction The first part of this element discusses the observations and results of the lab as well as any mistakes that may have been made (or what measures were taken to try to avoid mistakes) and what improvements, if any, you can bring forward of for the procedure for the next time the lab will be conducted. The discussion section of this lab should also include discussions on the following A comparison of how each solution (salt water and alcohol) garbled the hues from each color of candy or ink type.Which solution worked better at separating each of the pigments (it may be different for different candy colors or inks), and wherefore do you think that is? If you had allowed less time for the la b (stopping the separation process when the solution was half way up the paper) how do you think that would have affected your results? What pigment colors traveled farther up the paper? What pigment traveled the least? Was it the same or different when those pigments appeared in other candy colors or inks? Was it the same or different when separated by alcohol instead of salt water?